EGFR, KRAS, BRAF, MET, ERBB2 (Can be combined with MBS rebatable PDL1, ALK and ROS1 IHC/FISH) 

Covered by MBS items:  

• 73337 – EGFR  
• 73436 - MET exon 14 skipping 

If no variants are detected, RNA is tested to look for MET exon 14 skipping. 

If no drivers are identified by NGS then ROS1 IHC is performed.  

ALK and ROS1 fusions are confirmed by FISH. 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 
• IHC - fresh tissue or FFPE block/slides (15 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

EGFR – DNA only 

Fully covered (MBS item 73337) 

$337.75 (non-MBS eligible) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: <1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

Fully covered (MBS item 73436) 

$337.75 (non-MBS eligible) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

DNA only testing 

Sample taken after progression on EGFR TKI is required. 

Fully covered (MBS item 73351) 

$337.75 (non-MBS eligible) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: <1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) or digital droplet PCR (ddPCR) 

BRAF, NRAS, KIT, GNAQ, GNA11 

Fully covered (MBS item 73336) 

$196.35 (non-MBS eligible) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: <1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

KRAS, NRAS, BRAF, PIK3CA 

Fully covered (MBS item 73338) 

$308.25 (non-MBS eligible) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: <1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

KIT, PDGFRA, BRAF 

DNA only testing. 

If no variants are found by NGS, SDHB and panTRK IHC are performed. 

$350 (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 
• IHC - fresh tissue or FFPE block/slides (15 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

PIK3CA 

$350 (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: <1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

BRAF, NRAS, KRAS, RET, HRAS. 

$350 (not MBS covered). 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

BRAF, NRAS, KRAS, RET, HRAS plus RNA alterations 

$885 (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

$350 (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: <1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

$350 (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: <1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

Small DNA Variants - AKT1, AKT2, AKT3, ALK, AR, ARAF, BRAF, CDK4, CDKN2A, CHEK2, CTNNB1, EGFR, ERBB2, ERBB3, ERBB4, ESR1, FGFR1, FGFR2, FGFR3, FGFR4, FLT3, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, NTRK1, NTRK2, NTRK3, PDGFRA, PIK3CA, PTEN, RAF1, RET, ROS1, SMO, TP53. 

CNA – ALK, AR, CD274, CDKN2A, EGFR, ERBB2, ERBB3, FGFR1, FGFR2, FGFR3, KRAS, MET, PIK3CA, PTEN.  

Covers small DNA variants in targeted hotspot regions, and copy number alterations (CNAs) in selected genes. 

$600 DNA (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

RNA Fusions - ALK, BRAF, ESR1, FGFR1, FGFR2, FGFR3, MET, NRG1, NTRK1, NTRK2, NTRK3, NUTM1, RET, ROS1, RSPO2, RSPO3 

Splice Variants – AR-V7, EGFRvIII, and MET exon 14 skipping variants 

$600 RNA (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

Small DNA Variants - AKT1, AKT2, AKT3, ALK, AR, ARAF, BRAF, CDK4, CDKN2A, CHEK2, CTNNB1, EGFR, ERBB2, ERBB3, ERBB4, ESR1, FGFR1, FGFR2, FGFR3, FGFR4, FLT3, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, NTRK1, NTRK2, NTRK3, PDGFRA, PIK3CA, PTEN, RAF1, RET, ROS1, SMO, TP53. 

CNA – ALK, AR, CD274, CDKN2A, EGFR, ERBB2, ERBB3, FGFR1, FGFR2, FGFR3, KRAS, MET, PIK3CA, PTEN.  

RNA Fusions - ALK, BRAF, ESR1, FGFR1, FGFR2, FGFR3, MET, NRG1, NTRK1, NTRK2, NTRK3, NUTM1, RET, ROS1, RSPO2, RSPO3 

RNA Splice Variants – AR-V7, EGFRvIII, and MET exon 14 skipping variants 

Covers small DNA variants in targeted hotspot regions, copy number alterations, fusions and splice variants in selected genes. 

$885 RNA & DNA (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

Small DNA Variants - AKT1, AKT2, AKT3, ALK, AR, ARAF, BRAF, CDK4, CDKN2A, CHEK2, CTNNB1, EGFR, ERBB2, ERBB3, ERBB4, ESR1, FGFR1, FGFR2, FGFR3, FGFR4, FLT3, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, KIT, KRAS, MAP2K1, MAP2K2, MET, MTOR, NRAS, NTRK1, NTRK2, NTRK3, PDGFRA, PIK3CA, PTEN, RAF1, RET, ROS1, SMO, TP53. 

CNA – ALK, AR, CD274, CDKN2A, EGFR, ERBB2, ERBB3, FGFR1, FGFR2, FGFR3, KRAS, MET, PIK3CA, PTEN.  

$350 per gene (not MBS covered) 

TAT: 1 Week (from specimen receipt) 

Methodology: Oncomine Precision Assay (ThermoFisher Scientific) 

Fully covered (MBS item 73377) 

$212.50 (non-MBS eligible) 

Preferred samples:  

fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

• TAT: 2 Weeks (from specimen receipt) 

Methodology: Sanger sequencing looking for the c.402C>G p.(C134W) variant. 

Comprehensive genomic profiling for solid tumours including small sequence variants (523 genes), gene amplifications, gene fusions (55 genes), tumour mutation burden (TMB) and microsatellite instability (MSI). Contact mol path for further information.  

$2,500 (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

TAT: ~3 Weeks (from specimen receipt) 

BRCA1, BRCA2 

$600 (non-MBS eligible) 

Fully covered (MBS item 73303 - prostate) 

Fully covered (MBS item 73301 - ovarian) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

Methodology: NGS (QIAseq) 

$200 (not MBS covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

Methodology: Methylation Specific MLPA (MS-MLPA) & fragment analysis 

$300 (not MBS {slider=Accordion title}covered) 

Preferred samples:  

• fresh tissue or FFPE block/slides (10 x 5-micron sections on positively charged slides) 

Methodology: Methylation Specific MLPA (MS-MLPA) & fragment analysis   

$475 (not MBS eligible) 

*The V600E assay does not detect the DNA change c.1799_1800delinsAA, which also results in p.Val600Glu (V600E) also referred to as V600E2. 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$475 (not MBS eligible) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$525 (not MBS eligible) 

*The V600E assay does not detect the DNA change c.1799_1800delinsAA, which also results in p.Val600Glu (V600E) also referred to as V600E2. 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$475 (not MBS eligible) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$525 (not MBS eligible) 

EGFR activating variants available for testing: 

• c.2573T>G p.(Leu858Arg) 
• c.2156G>C p.(Gly719Ala) 
• c.2582T>A p.(Leu861Gln) 
• c.2235_2249del p.(Glu746_Ala750del) 
• c.2236_2250del p.(Glu746_Ala750del) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$475 (not MBS eligible) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$475 (not MBS eligible) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$475 (not MBS eligible) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$475 (not MBS eligible) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

$725 (not MBS eligible) 

TAT: 3 Business days  

Methodology: Digital droplet PCR (ddPCR) 

Next generation sequencing (NGS) liquid biopsy assay which includes analysis of small DNA sequence variants, selected gene fusions and gene amplifications (not clinically validated) in 77 genes. Contact mol path for further information. 

$3,500 (not MBS eligible) 

TAT: ~3 Weeks 

Sample requirements: 

Whole blood collected into 2x 10 mL Streck tubes. Note: collection technique impacts quality of test result, please contact mol path for further information.